Equipment

  • Cell Culture
    • Thermo Forma 3311 Incubator
    • Thermo Forma 3111 Incubator
    • Motic Microscope with Moticam camer

 

  • Microscopy
    • Nikon Ti-E microscope with
      • Motorized focus unit ( travel of 7.5mm upwards and 2.5mm down with a resolution of 0.025um)
      • Motorized stage ( travel of 110 x 75mm in 0.1um steps. Repeatability is +/- 0.5um with a maximum speed of 25mm/sec. )
      • Perfect Focus System (corrects focus drift during long-term observation and when reagents are added)
      • Objectives:
        • Plan Fluor 4x
        • Plan Fluor 10x
        • Plan Fluor 20x
        • Plan Fluor 40x.
        • All objectives are Phase Contrast Objectives. The 20x and 40x objectives are ELWD and have correction collars to allow for use with dishes of different thickness.
      • Filters:
        • Filter Cube 49000: ET-DAPI
          • Excitation: 350nm; Emission 460nm:
          • Suitable for: Alexa Fluor 350™, DAPI, DyLight 350, Hoechst 33258, LysoTracker Blue/MeOH, Blue Fluosphere (350/440)
        • Filter Cube 49002 : ET-GFP (FITC/Cy2)
          • Excitation:470nm ; Emission:525 nm
          • Suitable for: Acridine Orange + DNA,Azami Green,EGFP,Emerald GFP,GFP,LysoTracker Green/pH 5.2,MitoTracker Green FM/MeOH,mWasabi,SYBR® Green I,SYTO 9/DNA,wtGFP,ZsGreen1,5-(and 6)-carboxy-2′,7′-dichlorofluorescein,Alexa Fluor 488™,BODIPY FL/pH7.2,Calcium Green™-1,Cy2™,FAM,FITC,Fluo-3,Fluo-4,Oregon Green™ 488, Yellow-Green Fluospheres (505/515)
        • Filter Cube 49008: ET – mCherry, Texas Red
          • Excitation:560nm ; Emission630nm:
          • Suitable for: Alexa Fluor 568™,Alexa Fluor 594™,DyLight 594,LysoTracker Red/pH 5.2,mCherry,MitoTracker Deep Red 633/MeOH,mKate (TagFP635),mRFP1,ReAsH-CCPGCC,Rhodamine Red™-X,ROX,Sulforhodamine 101,Texas Red®,Texas Red®-X,X-rhod-1/Ca2+, Red Fluospheres (580/605)
      • Incubation Cabinet:
        • Microscope Box
        • Temperature Control Module
        • CO2 & Humidity Module
        • Micro-environmental chamber accommodating 6-12-24-48-96 well plates plus an insert for microscope slides and 35mm petri dishes
        • The temperature range of this system is: 3°C above Tamb÷50°C
      • Camera:
        • Andor Neo CMOS Camera
      • Software:
        • MetaMorph Acquisition and Analysis Software

 

  • PCR
    • StepOnePlus Real-Time PCR System from Applied BioSystems
    • Veriti 96-w Thermal Cycler from Applied Biosystems
    • FlashGel™ System for DNA, RNA & Recovery from Lonza

 

  • Spectrophotometers and Fluorospectrometers=
    • ND3300 Fluorospectrometer from nanodrop
    • NanoDrop ND1000 1ul Spectrophotometer from nanodrop

 

  • Microfluidics
    • Aladdin 2 syringe pumps
    • Ismatec IPC peristaltic pump
    • Zepto Plasma chamber from Henniker Plasma
    • Craft-ROBO CE5000-40-CRP

 

  • Mechanobiology
    •  BOSE BioDynamic® 5115 with torsion

 

  • Miscellaneous
    • pH and temperature probes: Oakton® Acorn™ Series pH 6 meter
    • Ice machine
    • Autoclave
    • Rocking shaker