Techniques

Traction Force Microscopy

In traction force microscopy, cells are seeded on a soft substrate in which fluorescent beads were embedded. As the cells move across the surface, they use the surface to pull themselves and displace the soft surface. This movement can be measured by recording the displacement of the fluorescent beads. Computing the mechanical properties of the substrate with the displacement of the beads allow us to calculate the strength of the cells.

Cells on gel
Fluorescent Beads
Traction Forces

Dynamic Cell Seeding

Using a custom microfluidic chamber, we are able to explore dynamic seeding in a bone scaffold. Until now, seeding in a scaffold was mainly achieved by dropping cells on the structure and letting them attach overnight. We are currently using a microfluidic chamber to explore seeding under oscillating flow in order to achieve better distribution and adhesion of cells in the scaffold.